rabbit polyclonal anti rps19  (Proteintech)

Journal: The EMBO Journal

Article Title: eIF3 mRNA selectivity profiling reveals eIF3k as a cancer‐relevant regulator of ribosome content

doi: 10.15252/embj.2022112362

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Article Snippet: Rabbit Polyclonal Anti‐ RPS19 (WB 1:2,000) , Bethyl , Cat # A304‐002A; RRID: AB_2620351.

Techniques: Recombinant, Plasmid Preparation, Sequencing, Protease Inhibitor, Magnetic Beads, Software, Microscopy, Microarray, Imaging, Flow Cytometry, Mutagenesis, Gel Extraction, cDNA Synthesis, Sample Prep, Clear Native PAGE, Bicinchoninic Acid Protein Assay

Journal: Molecular Cell

Article Title: Initiation of Quality Control during Poly(A) Translation Requires Site-Specific Ribosome Ubiquitination

doi: 10.1016/j.molcel.2016.11.039

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Article Snippet: Rabbit polyclonal anti-eS19 , Bethyl Labs , Cat. #A304-002A; RRID: AB_2620351.

Techniques: Recombinant, Protease Inhibitor, Methylation, Ubiquitin Proteomics, Expressing, Plasmid Preparation, Sequencing, Negative Control, Software

Journal: BBA Clinical

Article Title: Mutation of EMG1 causing Bowen–Conradi syndrome results in reduced cell proliferation rates concomitant with G2/M arrest and 18S rRNA processing delay

doi: 10.1016/j.bbacli.2014.05.002

Figure Lengend Snippet: Ribosomal subunit levels at steady-state are normal in BCS patient cells. (A, B) Sucrose gradient separation of 40S and 60S ribosomal subunits in lymphoblasts (A) and fibroblasts (B). Ribosomal subunits were detected following sucrose gradient separation by absorbance at 254 nm, and examples of the resultant tracings are shown. (C, D) Fractions were collected at regular intervals and probed by immunoblot analysis for ribosomal proteins using anti-RPS19 and anti-RPL7 antibodies to verify the separation of the small (RPS) and large (RPL) subunits. (E, F) The area under the curve was determined for individual peaks, and the ratio of large to small subunit was calculated. No significant difference between unaffected control and BCS-affected cells was found in lymphoblasts (E) and fibroblasts (F). The mean of six individual experiments and SEM are shown.

Article Snippet: Primary antibody dilutions were used as follows: RPL7 rabbit polyclonal at 1:5000 (Bethyl laboratories), RPS19 rabbit polyclonal at 1:3000 (Proteintech Group), TP53 2B2.68 mouse monoclonal at 1:2000 (Santa Cruz), p21/WAF1/Cip1 mouse monoclonal at 1:250 (Millipore), and β-actin mouse monoclonal at 1:5000 (Sigma).

Techniques: Western Blot, Control